Effects of (+)-pentazocine and 1,3-di-o-tolylguanidine (DTG), sigma (σ) ligands, on micturition in anaesthetized rats

摘要

The effects of two sigma (σ) binding site ligands, (+)-pentazocine and 1,3-di-o-tolylguanidine (DTG), on bladder functions were examined in rats.Cystometry using urethane-anaesthetized rats showed that (+)-pentazocine (1–5 mg kg−1, i.v.) and DTG (1–5 mg kg−1, i.v.) prolonged micturition intervals, indicating increased bladder capacity and raised the threshold pressure.The effects of (+)-pentazocine (2 mg kg−1, i.v.) on micturition were not influenced by naloxone (0.5 mg kg−1, i.v.), which antagonized similar effects of morphine (2 mg kg−1, i.v.).When administered intracerebroventricularly (i.c.v.), DTG (1 μg) and (+)-pentazocine (30 μg) prolonged micturition intervals with increased threshold pressure on the cystometrogram.In isolated bladder detrusor strips of rats, (+)-pentazocine (3 μM) and DTG (1 μM) did not affect contractile responses to electrical field stimulation. A higher concentration of DTG (3 μM) slightly suppressed the response induced by 30 Hz stimulation.The effects of (+)-pentazocine and DTG on micturition were abolished by pre-treatment with pertussis toxin (PTX, 1 μg, i.c.v.).These results indicate that typical σ ligands, such as (+)-pentazocine and DTG, increase bladder capacity in anaesthetized rats. Moreover, the mechanism by which σ ligands change the urinary storage properties in rats may involve pathways in which the function of Gi/o proteins is necessary.